Diamonsil™ AAA Columns

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Diamonsil® AAA Columns  


 

Diamonsil® AAA columns show outstanding selectivity and high resolution. Eighteen kinds of natural amino acids, an internal standard, and ammonia can be detected simultaneously using PITC and DNFB reagents.

 

The analysis method is stable and highly reproducible, and can therefore be used for quantitative as well as qualitative analysis. A variety of interfering factors such as reagents, byproducts, solvents, etc., can be removed by rapid extraction.

Every AAA column passes the test of separation of eighteen natural amino acids.

 

In proteomics and food quality tests, amino acid analysis (AAA) is often used as a tool to determine the exact composition of the amino acid

(AA) sample, so the improvement of amino acid analysis (AAA) is highly emphasized.

 

Diamonsil® AAA column is a new product made by Dikma Technologies Inc. which can perform amino acid analysis using two different amino acid derivative methods upon changing the chromatograph condition.

Eighteen Natural Amino Acids,Norleucine,and NH3-PITC Derivatives

Column:                          Diamonsil® 5µm AAA 250 x 4.6mm

Cat No:                           99751

Mobile phase:                   A:50 mM Sodium acetate(PH 6.50±0.05 adjusted with HCIO4)

                                      B:MeOH:MeCN:H2O=20:60:20(v/v/v)

Flow Rate:                       1.0mL/min

Temperature:                    45 oC

Injection Volumn:                   10µL

Detection:                                UV 254 nm

Gradient:

Time/min                0               39               40               45               46               60

A                            96             52               0                  0                 96               96

B                            5               48               100             100              5                 5
1.Asp 11.Tyr
2.Glu 12.Val
3.Ser 13.Met
4.Gly 14.Cys-Cys
5.His 15.lle
6.Arg 16.Leu
7.Thr 17.Nle
8.Ala 18.Phe
9.Pro 19.Trp
10.NH3 20.Lys
 

 

 

Eighteen Natural Amino Acids,2,4-Dinitrophenol,DNFB,Cysteine,2-Aminobutyric Acid,

and NH3-DNFB Derivatives

 

Column:                          Diamonsil® 5µm AAA 250 x 4.6mm

Cat No:                           99751

Mobile phase:                   A:20 mM Disodium hydrogen phosphate+20mM Sodium dihydrogen phosphate

                                      B:MeCN:MeOH=10:90(v/v)

Flow Rate:                       1.0mL/min

Temperature:                    35 oC

Injection Volumn:                   10µL

Detection:                                UV 360 nm

Gradient:

Time/min                0               39               44               44.01               65              

A                            86             60                30                  86                 86              

B                            14               40               70                14                  14               
1.Asp 12.Met
2.Glu 13.Cys-Cys
3.Ser 14.NH3
4.2,4-Dinitrophenol 15.lle
5.His 16.Leu
6.Arg 17Trp
7.Thr 18.His
8.Ala 19.Phe
9.Pro 20.DNFB
10.2-Aminobutytric acid 21.Cys
11.Val 22.Lys
  23.Tyr